143B細(xì)胞，ATCC人骨肉瘤細(xì)胞（CRL-8303）

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產(chǎn)品時間：2024-08-08
簡要描述：慧穎生物專業(yè)供應(yīng)143B細(xì)胞，ATCC人骨肉瘤細(xì)胞（CRL-8303）細(xì)胞系，成纖維細(xì)胞，提供的細(xì)胞狀態(tài)良好，飽滿，光澤度好?；鄯f生物擁有完善的細(xì)胞庫管理體系，售后及時到位。另有各類純進(jìn)口胎牛血清，培養(yǎng)基，雙抗、胰酶，DMSO，生長因子等相關(guān)培養(yǎng)產(chǎn)品，種類齊全，*。！

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產(chǎn)品簡介

產(chǎn)品名稱：143B細(xì)胞，人骨肉瘤細(xì)胞系

來源：ATCC

貨號：CRL-8303

器官來源：骨

生長狀態(tài)：貼壁生長

規(guī)格：株

運輸狀態(tài)：凍存或常溫

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143B細(xì)胞，ATCC人骨肉瘤細(xì)胞（CRL-8303），詳細(xì)培養(yǎng)資料及價格，請點擊：

來源：慧穎細(xì)胞庫

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細(xì)胞來源背景介紹：

Designations:	143B
Depositors:	Wistar Institute
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens
Morphology:	mixed
Source:	Organ: bone Disease: osteosarcoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host
DNA Profile (STR):	Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 11 vWA: 18
Age:	13 year old
Gender:	female
Ethnicity:	Caucasian
Comments:	Thymidine kinase negative (TK-). This is a human osteosarcoma cell line.
Propagation:	ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10% Temperature: 37.0°C
Subculturing:	Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Preservation:	90% FBS; 10% DMSO
Related Products:	derivative:ATCC CRL-8304
References:	32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 870925632519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 862775833047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 866275733152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651